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Mission Lentiviral Control Vectors

When conducting experiments using MISSION shRNA clones, proper controls are a key element of experimental design to permit accurate interpretation of knockdown results and provide assurance of the specificity of the response observed. The MISSION Control Vectors are lentiviral-based vectors that are useful as both positive and negative controls in experiments using the MISSION shRNA library. The DNA format controls may be used in direct transfection of target cells, or they may also be used to create replication-incompetent viral particles.

The control vectors listed below in the control selection table are held by the shRNA Shared Technology Resource. Further information on finding the appropriate controls for your experiments can be found at the Sigma Mission website. If you have questions, contact the core director. 

Control Selection Table
Catalog Number
Description
Vector Backbone Insert Insert Sequence / Vector Description 
SHC001
MISSION
pLKO.1-puro
Empty Vector
Control
TRC1/1.5 No hairpin No shRNA Insert
SHC002
MISSION
pLKO.1-puro
Non-Mammalian
shRNA
Control 
TRC1/1.5 Non human or
mouse shRNA
CCGGCAACAAGATGAAGAGCACCAACTC-GAGTTGGTGCTCTTCATCTTGTTGTTTTT
SHC003
MISSION
pLKO.1-puroCMVTurboGFP™
Positive Contro
TRC1/1.5 No hairpin No shRNA insert. Contains TurboGFP gene,
under the control of the CMV promoter.
TurboGFP is an improved variant of the green
fluorescent protein copGFP cloned from the
copepoda Pontellina plumata.
SHC201
MISSION TRC2
pLKO.5-
puro Empty
Vector Control
TRC2 No hairpin No shRNA Insert
SHC002
MISSION
pLKO.1-puro
Non-Mammalian
shRNA
Control
TRC2 Non human or
mouse shRNA
CCGGCAACAAGATGAAGAGCACCAACTC-GAGTTGGTGCTCTTCATCTTGTTGTTTTT
SHC203
MISSION TRC2
pLKO.5-
puro-CMVTurboGFP™
Positive Control
TRC2 No hairpin No shRNA insert. Contains TurboGFP gene,
under the control of the CMV promoter.
TurboGFP is an improved variant of the green
fluorescent protein copGFP cloned from the
copepoda Pontellina plumata.