When conducting experiments using MISSION shRNA clones, proper controls are a key element of experimental design to permit accurate interpretation of knockdown results and provide assurance of the specificity of the response observed. The MISSION Control Vectors are lentiviral-based vectors that are useful as both positive and negative controls in experiments using the MISSION shRNA library. The DNA format controls may be used in direct transfection of target cells, or they may also be used to create replication-incompetent viral particles.
The control vectors listed below in the control selection table are held by the shRNA Shared Technology Resource. Further information on finding the appropriate controls for your experiments can be found at the Sigma Mission website. If you have questions, contact the core director.
