IV Fluid Supply Updates

Cell & Molecular Imaging Equipment

Leica Stellaris 8 TauSTED Xtend

Drug Discovery – 521C: Confocal/Super-Resolution microscope for cutting-edge fluorescence imaging of live cells and fixed specimens.

  • Imaging modes include conventional confocal, LIGHTNING super-resolution, Stimulated Emission Depletion (STED) super-resolution, and FALCON (FAst Lifetime CONtrast) fluorescence lifetime imaging (FLIM).
  • Inverted microscope equipped with 10X air, 20X oil, 40X oil, 86X water, 93X glycerol, and 100X oil objectives.
  • White Light Laser with excitation from 440 nm up to 790 nm.
  • TauSTED powered by TauSense with three STED depletion laser lines: 592 nm, 660 nm, and 775 nm.
  • Five sensitive HyD S and HyD X detectors.
  • Brightfield imaging.
  • Other imaging modes include time-lapse, multi-track, depth, ratio, FRAP (fluorescence recovery after photo bleaching), and FRET (fluorescence resonance energy transfer).
  • Environmental chamber for temperature, humidity and CO2 control.

Leica STELLARIS STED Sample Preparation Guide (PDF)

Zeiss LSM 880 NLO

Drug Discovery - 521E: Confocal/multiphoton microscope for long-term fluorescence imaging of live cells, embryos, and tissues of living organisms as well as fixed cells and tissues.

  • Confocal fluorescence imaging using single and multiphoton laser excitation.
  • Axio Observer Z1 inverted microscope equipped with 10X air, 25X water, 40X water, 63X water, and 63X oil objectives.
  • Visible lasers for confocal excitation at 405, 458, 477, 488, 514, 561, and 633 nm.
  • Coherent Chameleon Ultra Ti: Sapphire multiphoton laser tunable from 690-1080 nm.
  • Multiphoton fluorescence imaging in both descanned and non-descanned modes.
  • Brightfield and DIC imaging using single and multiphoton excitation.
  • Spectral analysis and unmixing using Quasar 32-channel photomultiplier array.
  • GaAsP detectors for highest sensitivity imaging.
  • Fast Airyscan super-resolution detector.
  • Simultaneous 3-channel fluorescence imaging and DIC.
  • Other imaging modes include time-lapse, multi-track, intravital, depth, ratio, FLIM (fluorescence lifetime imaging), FRAP (fluorescence recovery after photobleaching), and FRET (fluorescence resonance energy transfer) and SHG (second harmonic generation).
  • Stage top incubator for temperature and CO2 control with objective heater.

Zeiss LSM 880 Operating Instructions

Olympus FV1200 MPE

Drug Discovery - 521D: Intravital multiphoton microscope for imaging live animals and thick specimens.

  • Olympus IX83 inverted microscope stand.
  • 4X and 10X air objectives plus 30X and 60X long working distance silicone oil immersion objectives.
  • Brightfield and DIC imaging using two photon excitation.
  • Spectra-Physics MaiTai DeepSee multiphoton laser tunable from 690-1040nm.
  • Simultaneous detection of four channels is possible with two conventional PMT detectors and two highly efficient GaAsP detectors.
  • Environmental chamber controls stage, temperature, and specimen atmosphere.
  • Second harmonic generation (SHG) imaging.

Olympus FV1200 User Manual (PDF)

Olympus FV1200 Operating Instructions

Andor BC43 

Drug Discovery - 521E: Benchtop spinning disk confocal microscope for fluorescence imaging of live cells and fixed tissues, 3D imaging of thick samples and large organism montages.

  • Inverted microscope equipped with 2X air, 10X air, 20X air, and 60X oil objectives.

  • Laser excitation at 405, 488, 561, and 638 nm.

  • Imaging speed above 30 frames per second.

  • Widefield imaging.

  • Transmitted light imaging includes brightfield mode and Differential Phase Contrast (DPC) mode.

  • Other imaging modes include time-lapse, multi-channel, Z-stack, multi-field, and montage.

  • 4.1 MP sCMOS camera with 15 fps full frame and ClearView GPU deconvolution.

  • Built-in anti-vibration.

  • Environmental chamber for temperature, humidity and CO2 control.

  • Come with the introductory Imaris analysis software.

Andor BC43 User Guide (PDF)

Zeiss LSM 510 META

Hollings Cancer Center - 504: Confocal microscope for fluorescence imaging of live cells and fixed specimens.

  • Confocal fluorescence imaging.
  • Axiovert 200M inverted microscope stand equipped with 20X, 40X oil, and 63X oil objectives.
  • Brightfield and DIC imaging.
  • Laser excitation at 405, 488, 514, 543, and 633 nm.
  • Spectral analysis and unmixing with META photomultiplier array.
  • Simultaneous multi-channel image acquisition with multi-tracking.
  • Other imaging modes include time-lapse, multi-track, depth, ratio, FRAP (fluorescence recovery after photobleaching), and FRET (fluorescence resonance energy transfer).
  • Stage top incubator for temperature and CO2 control with objective heater.

Zeiss LSM 510 User Manual (PDF)

Zeiss LSM 510 Operating Instructions (PDF)

Olympus FluoView FV10i LIV

Drug Discovery - 527A: Confocal microscope for automated long-term fluorescence imaging of live cells as well as fixed specimens.

  • 10X air and 60X water immersion objectives.
  • Phase contrast imaging.
  • Laser excitation at 405, 473, 559, and 635 nm.
  • Detection through a variable bandpass filter for selection of the most suitable wavelength for a particular fluorophore.
  • Environmental control for live cell imaging, including hypoxia control.
  • Z-drift compensation function for long-term imaging.

Olympus FV10i User Manual (PDF)

Olympus FV10i Operating Instructions (PDF)

BD Biosciences CARV II

Drug Discovery - 526: Disk-screening confocal microscope for brightfield and both widefield and confocal fluorescence imaging.

  • Zeiss Axiovert 200M inverted microscope stand.
  • 5X,10X, and 20X air and 40X and 60X oil immersion objectives.
  • Brightfield imaging of histochemical and immunohistochemical samples.
  • Spinning disk confocal attachment for video rate microscopy.

BD CARV II User Manual (PDF)

Brightfield Imaging Instructions (PDF)

Spinning Disc CARV II Operating Instructions (PDF)

Imaris

Drug Discovery – 502: Imaris 3/4D Image Visualization and Analysis software.

  • Render 3D/4D images, detect objects, snapshot & animation.

  • Report and interact with detected object measurements.

  • Visualize and quantify colocalized regions.

  • Plot in 1D-4D, compare groups with statistical tests.

  • Track motion in 2D/3D, detect divisions, create lineage tree.

  • Trace filamentous structures, neurons, vessels, detect spines.

  • Segment and analyze cells and their compartments.

  • Customize analysis with Matlab, Python, Java, R.

  • Utilize saved protocols for batch analysis.

  • GPU-accelerated deconvolution.

  • Precise alignment and stitching of multiple image tiles.

Huygens

Drug Discovery – 502: Huygens Image Deconvolution and Restoration Software Suite.

  • Deconvolution for Confocal, Widefield, Multi-photon, and Airyscan images.

  • Restoration options include Chromatic Aberration Corrector, Crosstalk Corrector, Tile Stitching, Object Stabilizer, PSF Distiller, and Deconvolution Express.

  • Visualization options include Movie Maker, Twin Slicer, Ortho Slicer, Surface Renderer, SFP Renderer, and Twin MIP Renderer.

  • Analysis options include Object Analyzer Advanced and Colocalization Analyzer.

  • Functionality options include Basic File Reader, Full File Reader, and Time Series Support.