Equipment

Zeiss 880 LSM NLO

Drug Discovery - 521E: Confocal/multiphoton microscope for long-term fluorescence imaging of live cells, embryos, and tissues of living organisms as well as fixed cells and tissues.

  • Confocal fluorescence imaging using single and multiphoton laser excitation.
  • Axio Observer Z1 inverted microscope equipped with 10X air, 25X water, 40X water, 63X water, and 63X oil objectives.
  • Visible lasers for confocal excitation at 405, 458, 477, 488, 514, 561, and 633 nm.
  • Coherent Chameleon Ultra Ti: Sapphire multiphoton laser tunable from 690-1080 nm.
  • Multiphoton fluorescence imaging in both descanned and non-descanned modes.
  • Brightfield and DIC imaging using single and multiphoton excitation.
  • Spectral analysis and unmixing using Quasar 32-channel photomultiplier array.
  • GaAsP detectors for highest sensitivity imaging.
  • Simultaneous 3-channel fluorescence imaging and DIC.
  • Other imaging modes include time-lapse, multi-track, intravital, depth, ratio, PLIM (phosphorescence lifetime imaging), FRAP (fluorescence recovery after photobleaching), and FRET (fluorescence resonance energy transfer) and SHG (second harmonic generation).
  • Stage top incubator for temperature and CO2 control with objective heater.

Zeiss LSM 880 Operating Instructions

Olympus FV1200 MPE

Drug Discovery - 521D: Intravital multiphoton microscope for imaging live animals and thick specimens.

  • Olympus IX83 inverted microscope stand.
  • 4X and 10X air objectives plus 30X and 60X long working distance silicone oil immersion objectives.
  • Brightfield and DIC imaging using two photon excitation.
  • Spectra-Physics MaiTai DeepSee multiphoton laser tunable from 690-1040nm.
  • Simultaneous detection of four channels is possible with two conventional PMT detectors and two highly efficient GaAsP detectors.
  • Environmental chamber controls stage, temperature, and specimen atmosphere.
  • Second harmonic generation (SHG) imaging.

Olympus FV1200 User Manual (PDF)

Olympus FV1200 Operating Instructions

Zeiss LSM 510 META

Hollings Cancer Center - 355: Confocal microscope for fluorescence imaging of live cells and fixed specimens.

  • Confocal fluorescence imaging.
  • Axiovert 200M inverted microscope stand equipped with 20X, 40X oil, and 63X oil objectives.
  • Brightfield and DIC imaging.
  • Laser excitation at 405, 488, 514, 543, and 633 nm.
  • Spectral analysis and unmixing with META photomultiplier array.
  • Simultaneous multi-channel image acquisition with multi-tracking.
  • Other imaging modes include time-lapse, multi-track, depth, ratio, FRAP (fluorescence recovery after photobleaching), and FRET (fluorescence resonance energy transfer).
  • Stage top incubator for temperature and CO2 control with objective heater.

Zeiss LSM 510 User Manual (PDF)

Zeiss LSM 510 Operating Instructions (PDF)

Olympus FluoView FV10i LIV

Drug Discovery - 512C: Confocal microscope for automated long-term fluorescence imaging of live cells as well as fixed specimens.

  • 10X air and 60X water immersion objectives.
  • Phase contrast imaging.
  • Laser excitation at 405, 473, 559, and 635 nm.
  • Detection through a variable bandpass filter for selection of the most suitable wavelength for a particular fluorophore.
  • Environmental control for live cell imaging, including hypoxia control.
  • Z-drift compensation function for long-term imaging.

Olympus FV10i User Manual (PDF)

Olympus FV10i Operating Instructions (PDF)

BD Biosciences CARV II

Drug Discovery - 526: Disk-screening confocal microscope for brightfield and both widefield and confocal fluorescence imaging.

  • Zeiss Axiovert 200M inverted microscope stand.
  • 5X,10X, and 20X air and 40X and 60X oil immersion objectives.
  • Brightfield imaging of histochemical and immunohistochemical samples.
  • Spinning disk confocal attachment for video rate microscopy.

BD CARV II User Manual (PDF)

Brightfield Imaging Instructions (PDF)

Spinning Disc CARV II Operating Instructions (PDF)