Center for Cellular Therapy

Staff

Associate Director

Shikhar Mehrotra, Ph.D.
Associate Professor, Surgery
mehrotr@musc.edu

Operations Manager

Colleen Cloud
cloud@musc.edu
843-792-9269

If you have a research initiative and would like to use the Center for Cellular Therapy, please contact us at cellular-therapy@musc.edu.

Location

Clinical Sciences Building
2nd Floor

Services

Generation of cGMP Products

The Center for Cellular Therapy staff has extensive experience in all aspects of cellular therapy and cGMP Phase I/II manufacturing of autologous cellular products. These include cGMP handling of blood components; dendritic cells, mesenchymal stem cells, T-cell & immunotherapy expertise; and clinical experience in transplantation and cellular therapies. In addition to technical expertise, extensive QA/QC and regulatory experience are available to ensure FDA compliance during the development of each specific cellular product.

Staff can provide assistance in the development of standard operating procedures, performance of process and/or equipment validation, staff training, and competency/proficiency assessments, and preparation of regulatory documents including IND applications.

The Center for Cellular Therapy is dedicated to scaling up translational studies within Hollings Cancer Center and supporting investigator-initiated clinical trials involving cellular therapy. Support includes technology transfer from the research laboratory to the clinical setting as well as scale-up and validation studies.

Immune Monitoring

Immunomonitoring entails characterization and quantification of immune cells (lymphocytes, monocytes, dendritic cells (DC)), identification of "rare" sub-population of cells (e.g., flow cytometry techniques combining multiple parameters), and the study of complex immunological functions (cell proliferation, antigen presentation, synthesis of cytokines, antibodies or other biomarkers, gene expression profiles).

For example, the DC validation essential for conducting DC clinical trials involves characterization of the DC phenotype using the cell surface markers CD14, CD80, CD86 and HLA-DR. The PBMCs collected at different time points are evaluated quantitatively for tumor epitope specific T-cells using tetramer staining, ELISPOT, and qualitatively by ELISA. For studies examining mesenchymal stem cells, suppressive functions of the MSCs are tested in a suppression assay that determines the proliferation of CD3 T-cells in the presence or absence of MSCs at various E:T ratio.

For more information on all of these services, please visit the Center for Cellular Therapy.

Publication Acknowledgement

If you publish a manuscript including research supported by the Cell Evaluation & Therapy Shared Resource, the HCC recommends the following text to be placed in the acknowledgement section: "Supported in part by the Cell Evaluation & Therapy Shared Resource, Hollings Cancer Center, Medical University of South Carolina (P30 CA138313)."