Flow Cytometry & Cell Sorting Shared Resource

Staff

Associate Director

Amanda C. LaRue, Ph.D.
Associate Professor, Pathology & Laboratory Medicine
laruerc@musc.edu

Operations Manager

B. Jacob Kendrick, MS
Hollings Cancer Center – 309 
kendricb@musc.edu
843-792-9602

Mass Cytometry Operations Manager

Carsten Krieg, Ph.D.
Assistant Professor, Microbiology & Immunology and Dermatology
kriegc@musc.edu
843-792-9995

Research Specialist

Corinne Levingston
schuylec@musc.edu
843-792-8115
Hollings Cancer Center – 324

Research Specialist

Kirsten McDanel
mcdanelk@musc.edu
843-792-8115
Hollings Cancer Center – 324

Services

User-based (unassisted) Flow Cytometric Analysis

Equipment is available 24/7 for trained investigators. During regular working hours, operators are available to assist with setup, running samples, and troubleshooting.

Assisted Flow Cytometric Analysis

An operator is available for the Fortessa X-20, LSRFortessa, and FACSVerse during regular working hours to provide general assistance as well as standard assays including cell death, proliferation, cell cycle, Ca++ flux, etc.

Imaging Flow Cytometry

The FCCS offers imaging flow cytometry as an assisted-use service on the Luminex ImageStream. The ImageStreamX Mk II System is a benchtop, multispectral, imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery, allowing users to locate and quantify the distribution of signals on, within, or between cells.

Cell Sorting

The FCCS offers high-speed cell sorting services for isolation of complex and rare cell populations. The staff of the FCCS has considerable expertise in the design and implementation of novel methodologies for cell/particle isolation and is available to work with investigators to support study goals. Cell sorting is provided as an operator-based assisted service.

Mass Cytometry

The FCCS provides mass cytometry services for high-dimensional single-cell analysis. The Helios (CyTOF) platform is capable of simultaneous analysis of up to 135 antigens, with current studies detecting 45-50 parameters. Dr. Krieg provides expertise in experimental design, data analysis, and troubleshooting for CyTOF experiments. Mass cytometry analysis is provided as an operator-based assisted service. 

Additional Information & Helpful Links

Publication Acknowledgement

If you publish a manuscript including research supported by the Flow Cytometry & Cell Sorting Shared Resource, Hollings Cancer Center recommends the following text to be placed in the acknowledgement section: "Supported in part by the Cell Evaluation & Therapy Shared Resource, Hollings Cancer Center, Medical University of South Carolina (P30 CA138313)."