Flow Cytometry & Cell Sorting Shared Resource


Associate Director

Amanda C. LaRue, Ph.D.
Associate Professor, Pathology & Laboratory Medicine

Operations Manager

B. Jacob Kendrick, MS
Hollings Cancer Center – 309 

Mass Cytometry Operations Manager

Carsten Krieg, Ph.D.
Assistant Professor, Microbiology & Immunology and Dermatology

Research Specialist

Corinne Levingston
Hollings Cancer Center – 324

Research Specialist

Kirsten McDanel
Hollings Cancer Center – 324


User-based (unassisted) Flow Cytometric Analysis

Equipment is available 24/7 for trained investigators. During regular working hours, operators are available to assist with setup, running samples, and troubleshooting.

Assisted Flow Cytometric Analysis

An operator is available for the Fortessa X-20, LSRFortessa, and FACSVerse during regular working hours to provide general assistance as well as standard assays including cell death, proliferation, cell cycle, Ca++ flux, etc.

Imaging Flow Cytometry

The FCCS offers imaging flow cytometry as an assisted-use service on the Luminex ImageStream. The ImageStreamX Mk II System is a benchtop, multispectral, imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery, allowing users to locate and quantify the distribution of signals on, within, or between cells.

Cell Sorting

The FCCS offers high-speed cell sorting services for isolation of complex and rare cell populations. The staff of the FCCS has considerable expertise in the design and implementation of novel methodologies for cell/particle isolation and is available to work with investigators to support study goals. Cell sorting is provided as an operator-based assisted service.

Mass Cytometry

The FCCS provides mass cytometry services for high-dimensional single-cell analysis. The Helios (CyTOF) platform is currently capable of simultaneously analyzing 50 parameters. Mass cytometry acquisition is provided as an operator-based assisted service only. Please contact Dr. Krieg for scheduling sample dropoffs. Under Helpful Links below, you will find some entry points for high-dimensional data analysis. 

Additional Information & Helpful Links

Publication Acknowledgement

If you publish a manuscript including research supported by the Flow Cytometry & Cell Sorting Shared Resource, Hollings Cancer Center recommends the following text to be placed in the acknowledgement section: "Supported in part by the Cell Evaluation & Therapy Shared Resource, Hollings Cancer Center, Medical University of South Carolina (P30 CA138313)."