Lipidomics Shared Resource

The Lipidomics Shared Resource (LSR) at MUSC Hollings Cancer Center represents expertise specializing in sphingolipid biology, providing services to MUSC and institutions and industries throughout the world. The Lipidomics Shared Resource is composed of analytical and synthesis units that build on the unique expertise of key personnel in sphingolipid chemistry, analysis, and biology providing intellectual and physical resources to enhance the understanding of bioactive sphingolipids and lipids in signal transduction and cell regulation.

Sphingolipids are recognized as key components regulating fundamental cell biology processes regulating transmembrane signaling. Diversity of bioactive sphingolipids and their interconnected metabolism provide a network of pathways. Key sphingolipid metabolites are: ceramides (Cer), sphingosine (Sph), sphingosine 1-phosphate (S1P), and ceramide 1-phosphate (Cer1P).

Staff

Director

Besim Ogretmen, Ph.D.
Professor, Biochemistry and Molecular Biology
ogretmen@musc.edu
843-792-0940

MALDI Imaging Unit Operations Manager

Peggi Angel, Ph.D.
angelp@musc.edu
843-792-8410

Analytical Unit Operations Manager

Jason S. Pierce, MS
piercej@musc.edu
843-792-2495

Synthesis Unit Operations Manager

Zdzislaw Szulc, Ph.D.
sculcz@musc.edu
843-792-0755

Services & Expertise

The Lipidomics Shared Resource is designed to support research in lipidomics by providing advanced methodology and expertise for both synthesis and analysis of bioactive lipids in a cost-effective manner, with a focus on several key areas:

• Providing qualitative and quantitative analysis of >300 sphingolipids using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) technologies.
• Providing analytical method development of new lipid components.
• Providing and developing synthetic lipid tools/standards, including functionalized and fluorescent ceramides, 17C-sphingolipids, or synthetic lipid analogs, including organelle-targeted sphingolipids.
• Mentoring investigators in the understanding of lipid biology through lipid chemistry, experimental design, selection of lipids of interest, and interpretation of the analytical data.

Publication Acknowledgement

The following text must be placed under the acknowledgements section of publications:

“Supported in part by the Lipidomics Shared Resource, Hollings Cancer Center, Medical University of South Carolina (P30 CA138313 and P30 GM103339)”.

Publications

The following publications are a result of services provided by the Lipidomics Shared Resource:

1. Spassieva SD, et al., Ectopic expression of ceramide synthase 2 in neurons suppresses neurodegeneration induced by ceramide synthase 1 deficiency. Proc Natl Acad Sci U S A. 2016 May 24;113(21):5928-33.
2. Simanshu DK et al., Arabidopsis accelerated cell death 11, ACD11, is a ceramide-1-phosphate transfer protein and intermediary regulator of phytoceramide levels. Cell Rep. 2014 Jan 30;6(2):388-99.
3. Neviani P et al., PP2A-activating drugs selectively eradicate TKI-resistant chronic myeloid leukemic stem cells. J Clin Invest. 2013 Oct;123(10):4144-57.
4. Sentelle RD et al., Ceramide targets autophagosomes to mitochondria and induces lethal mitophagy. Nat Chem Biol. 2012 Oct;8(10):831-8.
5. Chipuk JE et al., Sphingolipid metabolism cooperates with BAK and BAX to promote the mitochondrial pathway of apoptosis. Cell. 2012 Mar 2;148(5):988-1000.
6. D'Angelo G et al., Glycosphingolipid synthesis requires FAPP2 transfer of glucosylceramide. Nature. 2007 Sep 6;449(7158):62-7.